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发明公开
EP2749638A4 TRANSFORMANT OF CORYNEFORM BACTERIUM AND METHOD FOR PRODUCING VALINE BY USING SAME 审中-公开
MITTEL ZUR TRANSFORMIERUNG CORYNEFORMER BAKTERIEN SOWIE VERFAHREN ZUR VALINHERSTELLUNG DAMIT

TRANSFORMANT OF CORYNEFORM BACTERIUM AND METHOD FOR PRODUCING VALINE BY USING SAME
摘要：
A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host. (a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof. (b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof. (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or an analog thereof.
摘要（中）：

将一种或多种以下DNA（a），（b）和（c）引入作为宿主的棒状细菌获得的转化体。 （a）编码源自谷氨酸棒杆菌的乙酰羟酸合成酶的DNA，其具有在DNA编码的氨基酸序列中将位置156处的甘氨酸变为谷氨酸（G156E）的突变或其类似物。 （b）编码源自谷氨酸棒杆菌的乙酰羟酸异构体还原酶的DNA，其具有将位置34处的丝氨酸改变为甘氨酸（S34G），将第48位的亮氨酸变为谷氨酸（L48E），将第49位的精氨酸变为苯丙氨酸的突变 （R49F）在由DNA编码的氨基酸序列中，或其类似物。 （c）编码来源于裂殖杆菌的亮氨酸脱氢酶的DNA或其类似物。

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