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    DNA FRAGMENT HAVING PROMOTER FUNCTION
    3.
    发明公开
    DNA FRAGMENT HAVING PROMOTER FUNCTION 审中-公开
    DNA-FRAGMENT MIT PROMOTORFUNKTION

    公开(公告)号：EP1790721A4

    公开(公告)日：2008-12-10

    申请号：EP05776785

    申请日：2005-09-05

    申请人： RES INST INNOVATIVE TECH EARTH

    发明人： YUKAWA HIDEAKI ,  INUI MASAYUKI

    IPC分类号： C12N15/09 ,  C12N1/20

    CPC分类号： C07K14/34 ,  C12N15/67

    摘要： To produce a useful organic compound under anaerobic conditions, it is intended to provide a method of inducing the expression of the promoter functions of various genes in a coryneform bacterium participating in the exertion of the functions to thereby highly efficiently exert the functions of the coryneform bacterium under such conditions. More specifically speaking, it is intended to efficiently and highly express various protein genes necessary in producing a target substance while inhibiting the expression of unnecessary protein genes by providing a method of enhancing and/or inhibiting the promoter functions relating to the above-described genes. A DNA fragment which is useful as a primer to be transferred into a coryneform bacterium capable of highly efficiently producing a useful substance such as lactic acid or succinic acid at a high yield.

    TRANSFORMANT OF CORYNEFORM BACTERIUM AND METHOD FOR PRODUCING VALINE BY USING SAME
    7.
    发明公开
    TRANSFORMANT OF CORYNEFORM BACTERIUM AND METHOD FOR PRODUCING VALINE BY USING SAME 审中-公开
    MITTEL ZUR TRANSFORMIERUNG CORYNEFORMER BAKTERIEN SOWIE VERFAHREN ZUR VALINHERSTELLUNG DAMIT

    公开(公告)号：EP2749638A4

    公开(公告)日：2015-04-22

    申请号：EP12826287

    申请日：2012-08-21

    申请人： RES INST INNOVATIVE TECH EARTH

    发明人： YUKAWA HIDEAKI ,  INUI MASAYUKI

    IPC分类号： C12N1/21 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/09 ,  C12P13/08 ,  C12R1/15

    CPC分类号： C12N15/77 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/1022 ,  C12P13/08 ,  C12Y101/01086 ,  C12Y104/01009 ,  C12Y202/01006 ,  Y02P20/52

    摘要： A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host. (a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof. (b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof. (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or an analog thereof.

    摘要翻译： 将一种或多种以下DNA（a），（b）和（c）引入作为宿主的棒状细菌获得的转化体。 （a）编码源自谷氨酸棒杆菌的乙酰羟酸合成酶的DNA，其具有在DNA编码的氨基酸序列中将位置156处的甘氨酸变为谷氨酸（G156E）的突变或其类似物。 （b）编码源自谷氨酸棒杆菌的乙酰羟酸异构体还原酶的DNA，其具有将位置34处的丝氨酸改变为甘氨酸（S34G），将第48位的亮氨酸变为谷氨酸（L48E），将第49位的精氨酸变为苯丙氨酸的突变 （R49F）在由DNA编码的氨基酸序列中，或其类似物。 （c）编码来源于裂殖杆菌的亮氨酸脱氢酶的DNA或其类似物。