发明公开
- 专利标题： MEASUREMENT OF BIOLOGICALLY LABILE HYDROGEN SULFIDE POOLS
- 专利标题（中）： 测量生物制氢不稳定藏的
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申请号： EP13767904.9申请日： 2013-03-14
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公开(公告)号： EP2831581A1公开(公告)日： 2015-02-04
- 发明人: KEVIL, Christopher, G. , SHEN, Xinggui , PETER, Elvis, A.
- 申请人： Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College
- 申请人地址： LSU Health Science Center-Shreveport P.O. Box 33932 Shreveport, LA 71130-3932 US
- 专利权人： Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College
- 当前专利权人： Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College
- 当前专利权人地址： LSU Health Science Center-Shreveport P.O. Box 33932 Shreveport, LA 71130-3932 US
- 代理机构： WP Thompson
- 优先权： US201261617995P 20120330
- 国际公布： WO2013148246 20131003
- 主分类号： G01N33/48
- IPC分类号： G01N33/48 ; G01N33/483
摘要：
A method to measure all relevant biologic hydrogen sulfide pools, namely free hydrogen sulfide, acid-labile sulfide, and bound sulfane sulfur, has been developed. This new method involves selective liberation, trapping and derivatization of labile hydrogen sulfide. The total labile sulfide, including the contribution of the bound sulfane sulfur pool, the acid-labile pool, and free H2S, was measured by incubating the sample with a reducing agent, TCEP (Tris(2-carboxyethyl)phosphine hydrochloride), to reduce disulfide bonds in an acid solution. This method was used to measure the three sulfide pools in blood samples from mice and from humans. This method can be used for research, environmental, and clinical diagnostic purposes in determining hydrogen sulfide bioavailability in biological or other samples.
公开/授权文献
- EP2831581B1 MEASUREMENT OF BIOLOGICALLY LABILE HYDROGEN SULFIDE POOLS 公开/授权日：2018-10-03
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