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公开(公告)号：EP2979086B1
公开(公告)日：2017-11-08
申请号：EP14772704.4
申请日：2014-03-28
申请人： Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College , Louisiana Tech University Foundation
CPC分类号： G01N33/0044 , G01N27/40 , G01N31/223 , G01N33/0013 , G01N33/4925
摘要： A hydrogen sulfide (H2S) detecting apparatus for measuring the concentrations of hydrogen sulfide species in a given sample is disclosed. The hydrogen sulfide detecting apparatus can comprise a plurality of reaction chambers separated from a plurality of trapping chambers by a (H2S) permeable membrane, with the reaction chambers and trapping chambers each having buffer component(s) and/or reactive agents that expose the incoming sample to a particular pH and chemical environment in order to allow for the selective liberation and trapping of hydrogen sulfide from the sample.
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公开(公告)号：EP2831581B1
公开(公告)日：2018-10-03
申请号：EP13767904.9
申请日：2013-03-14
IPC分类号： G01N33/48 , G01N33/483 , G01N33/84
CPC分类号： G01N33/50 , G01N21/64 , G01N33/84 , G01N2021/7786 , Y10T436/184
摘要： A method to measure all relevant biologic hydrogen sulfide pools, namely free hydrogen sulfide, acid-labile sulfide, and bound sulfane sulfur, has been developed. This new method involves selective liberation, trapping and derivatization of labile hydrogen sulfide. The total labile sulfide, including the contribution of the bound sulfane sulfur pool, the acid-labile pool, and free H2S, was measured by incubating the sample with a reducing agent, TCEP (Tris(2-carboxyethyl)phosphine hydrochloride), to reduce disulfide bonds in an acid solution. This method was used to measure the three sulfide pools in blood samples from mice and from humans. This method can be used for research, environmental, and clinical diagnostic purposes in determining hydrogen sulfide bioavailability in biological or other samples.
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公开(公告)号：EP2979086B8
公开(公告)日：2018-03-28
申请号：EP14772704.4
申请日：2014-03-28
申请人： Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College , Louisiana Tech University
CPC分类号： G01N33/0044 , G01N27/40 , G01N31/223 , G01N33/0013 , G01N33/4925
摘要： A hydrogen sulfide (H2S) detecting apparatus for measuring the concentrations of hydrogen sulfide species in a given sample is disclosed. The hydrogen sulfide detecting apparatus can comprise a plurality of reaction chambers separated from a plurality of trapping chambers by a (H2S) permeable membrane, with the reaction chambers and trapping chambers each having buffer component(s) and/or reactive agents that expose the incoming sample to a particular pH and chemical environment in order to allow for the selective liberation and trapping of hydrogen sulfide from the sample.
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公开(公告)号：EP2979086A1
公开(公告)日：2016-02-03
申请号：EP14772704.4
申请日：2014-03-28
申请人： Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College , Louisiana Tech University Foundation
IPC分类号： G01N31/00
CPC分类号： G01N33/0044 , G01N27/40 , G01N31/223 , G01N33/0013 , G01N33/4925
摘要： A hydrogen sulfide (H
2 S) detecting apparatus for measuring the concentrations of hydrogen sulfide species in a given sample is disclosed. The hydrogen sulfide detecting apparatus can comprise a plurality of reaction chambers separated from a plurality of trapping chambers by a (H
2 S) permeable membrane, with the reaction chambers and trapping chambers each having buffer component(s) and/or reactive agents that expose the incoming sample to a particular pH and chemical environment in order to allow for the selective liberation and trapping of hydrogen sulfide from the sample.-
公开(公告)号：EP2831581A1
公开(公告)日：2015-02-04
申请号：EP13767904.9
申请日：2013-03-14
IPC分类号： G01N33/48 , G01N33/483
CPC分类号： G01N33/50 , G01N21/64 , G01N33/84 , G01N2021/7786 , Y10T436/184
摘要： A method to measure all relevant biologic hydrogen sulfide pools, namely free hydrogen sulfide, acid-labile sulfide, and bound sulfane sulfur, has been developed. This new method involves selective liberation, trapping and derivatization of labile hydrogen sulfide. The total labile sulfide, including the contribution of the bound sulfane sulfur pool, the acid-labile pool, and free H2S, was measured by incubating the sample with a reducing agent, TCEP (Tris(2-carboxyethyl)phosphine hydrochloride), to reduce disulfide bonds in an acid solution. This method was used to measure the three sulfide pools in blood samples from mice and from humans. This method can be used for research, environmental, and clinical diagnostic purposes in determining hydrogen sulfide bioavailability in biological or other samples.
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