发明公开
- 专利标题： METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELL, COMPOSITION USED IN METHOD, AND USES THEREOF
- 专利标题（中）： 用于生产诱导多能干细胞，其组成及其用途
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申请号： EP14862407.5申请日： 2014-11-12
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公开(公告)号： EP3070163A1公开(公告)日： 2016-09-21
- 发明人: PEI, Duanqing , LIU, Jing , CHEN, Jiekai , PENG, Meixiu
- 申请人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences
- 申请人地址： 190 Kai Yuan Avenue Science Park Guangzhou, Guangdong 510530 CN
- 专利权人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences
- 当前专利权人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences
- 当前专利权人地址： 190 Kai Yuan Avenue Science Park Guangzhou, Guangdong 510530 CN
- 代理机构： Brown, David Leslie
- 优先权： CN201310574447 20131115
- 国际公布： WO2015070756 20150521
- 主分类号： C12N5/074
- IPC分类号： C12N5/074 ; C07K14/435
摘要：
Provided are a method for preparing an induced pluripotent stem cell and a composition used in the method. The method comprises: introducing a composition for promoting the formation of an induced pluripotent stem cell into a somatic cell, the composition comprising: (i) a c-Jun antagonist and one group of factors from among the following seven such groups: (1) Sox2, Klf4 and c-Myc, (2) Klf4 and c-Myc, (3) Oct3/4, Klf4 and c-Myc, (4) Sox2, Nanog and Lin28, (5) Oct3/4, Nanog and Lin28, (6) Oct3/4, Klf and Sox2, and (7) Klf4 and Sox2; or (ii) the c-Jun antagonist, Jhdm1b and Id1, and at least one of Glis1, Sall4 or Lrh1; or (iii) the c-Jun antagonist, Jhdm1b and Id1, and at least one of: Oct4, Klf4, Sox2, Lin28, Esrrb, Lef1, Utf1 or miRNA C. The present method allows for successful preparation of induced pluripotent stem cells with no generation of abnormal chromosomes.
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