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    METHOD FOR INTRODUCING EXOGENOUS MITOCHONDRIA INTO MAMMALIAN CELLS
    3.
    发明公开
    METHOD FOR INTRODUCING EXOGENOUS MITOCHONDRIA INTO MAMMALIAN CELLS 审中-公开
    VERFAHREN ZUREINFÜHRUNGVON葡萄糖素在SÄUGETIERZELLEN

    公开(公告)号：EP3067416A1

    公开(公告)日：2016-09-14

    申请号：EP14860811.0

    申请日：2014-08-25

    申请人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences

    发明人： LIU, Xingguo ,  PEI, Duanqing ,  LIU, Jinglei ,  LIU, Xuebin ,  YAO, Deyang

    IPC分类号： C12N5/10 ,  C12N15/85 ,  C12N5/071

    CPC分类号： C12N5/0645 ,  C12N15/87 ,  C12N2510/00

    摘要： The present disclosure provides a method for producing a cell with exogenous mitochondria by obtaining synthetic mitochondria via introduction of exogenous mitochondrial DNA into mitochondria or empty mitochondrial shells, and incorporating the same into mammalian cells via endocytosis. As such, effective functionality of exogenous mitochondria in cells is realized. The synthetic mitochondrial DNA genes introduced according to the present disclosure can be stably expressed and effectively passaged. The method for introducing exogenous mitochondrial DNA into mammalian cells as disclosed herein may be used as a whole new mitochondrial molecular cloning means to perform site-directed mutagenesis, gene insertion, gene knockout, gene rearrangement, and the like in mitochondria. Therefore, any molecular cloning modification can be performed on a mammalian mitochondrial DNA, which is of great importance to therapeutic schemes of diseases derived from mitochondrial DNA mutations.

    摘要翻译： 本公开内容提供了通过将外源性线粒体DNA引入线粒体或空线粒体壳而获得合成线粒体并通过内吞作用将其并入哺乳动物细胞的方式来制备具有外源性线粒体的细胞的方法。 因此，实现了细胞中外源性线粒体的有效功能。 根据本公开引入的合成线粒体DNA基因可以稳定表达并有效传代。 如本文所公开的，将外源线粒体DNA引入哺乳动物细胞的方法可以用作线粒体中进行定点诱变，基因插入，基因敲除，基因重排等的全新的线粒体分子克隆方法。 因此，可以对哺乳动物线粒体DNA进行任何分子克隆修饰，这对于源自线粒体DNA突变的疾病的治疗方案是非常重要的。

    METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELL, COMPOSITION USED IN METHOD, AND USES THEREOF
    6.
    发明公开
    METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELL, COMPOSITION USED IN METHOD, AND USES THEREOF 审中-公开
    用于生产诱导多能干细胞，其组成及其用途

    公开(公告)号：EP3070163A1

    公开(公告)日：2016-09-21

    申请号：EP14862407.5

    申请日：2014-11-12

    申请人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences

    发明人： PEI, Duanqing ,  LIU, Jing ,  CHEN, Jiekai ,  PENG, Meixiu

    IPC分类号： C12N5/074 ,  C07K14/435

    CPC分类号： C12N5/0696 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02

    摘要： Provided are a method for preparing an induced pluripotent stem cell and a composition used in the method. The method comprises: introducing a composition for promoting the formation of an induced pluripotent stem cell into a somatic cell, the composition comprising: (i) a c-Jun antagonist and one group of factors from among the following seven such groups: (1) Sox2, Klf4 and c-Myc, (2) Klf4 and c-Myc, (3) Oct3/4, Klf4 and c-Myc, (4) Sox2, Nanog and Lin28, (5) Oct3/4, Nanog and Lin28, (6) Oct3/4, Klf and Sox2, and (7) Klf4 and Sox2; or (ii) the c-Jun antagonist, Jhdm1b and Id1, and at least one of Glis1, Sall4 or Lrh1; or (iii) the c-Jun antagonist, Jhdm1b and Id1, and at least one of: Oct4, Klf4, Sox2, Lin28, Esrrb, Lef1, Utf1 or miRNA C. The present method allows for successful preparation of induced pluripotent stem cells with no generation of abnormal chromosomes.

    摘要翻译： 提供的是用于制备诱导的多能干细胞的组合物和该方法中使用的方法。 该方法包括：将组合物用于促销婷诱导的多能干细胞的形成成体细胞，该组合物包括：（i）一个c-Jun的拮抗剂和一种基团从以下七个寻求组间因素：（1） SOX2，KLF4和c-Myc，（2）Klf4和c-Myc的，（3）的Oct3 / 4，Klf4和c-Myc的，（4）的Sox2，Nanog和Lin28的，（5）的Oct3 / 4，Nanog和Lin28的， （6）的Oct3 / 4，Sox2和KLF，和（7）Klf4和Sox2的; 或（ii）所述的c-Jun拮抗剂，Jhdm1b和ID1，和GLIS1，Sall4中或LRH1中的至少一个; 或（iii）所述的c-Jun拮抗剂，Jhdm1b和ID1，和中的至少一个：OCT4，KLF4，SOX2，LIN28基因Esrrb，LEF-1，UTF1或miRNA C.本发明的方法允许对成功制备的诱导多能干细胞与 不产生异常染色体。

    METHOD FOR INCREASING EFFICIENCY OF GENERATING INDUCTED PLURIPOTENT STEM CELL
    9.
    发明公开
    METHOD FOR INCREASING EFFICIENCY OF GENERATING INDUCTED PLURIPOTENT STEM CELL 有权
    增产的效率的方法，诱导多能干细胞

    公开(公告)号：EP2647700A1

    公开(公告)日：2013-10-09

    申请号：EP12801798.5

    申请日：2012-03-31

    申请人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences

    发明人： PEI, Duanqing ,  WANG, Tao

    IPC分类号： C12N5/10 ,  C12N5/071

    CPC分类号： C12N5/0662 ,  C12N5/0696 ,  C12N2500/38 ,  C12N2501/602 ,  C12N2501/72 ,  C12N2506/13 ,  C12N2510/00

    摘要： The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells, and more particularly, to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1b and Jhdm1a that modify histone. By utilizing Jhdmlb, Jhdmla, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method of the present invention further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission.

    CULTURE MEDIUM ADDITIVE AND USES THEREOF
    10.
    发明公开
    CULTURE MEDIUM ADDITIVE AND USES THEREOF 审中-公开
    VERWENDUNGEN DAVON的KULTURMEDIUMZUSATZSTOFF

    公开(公告)号：EP2565263A1

    公开(公告)日：2013-03-06

    申请号：EP10850541.3

    申请日：2010-07-29

    申请人： Guangzhou Institute Of Biomedicine And Health, Chinese Academy Of Sciences

    发明人： PEI, Duanqing ,  CHEN, Jiekai ,  LIU, Jing

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/07

    CPC分类号： C12N5/0696 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/33 ,  C12N2501/603 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/1307 ,  C12N2510/00

    摘要： The present invention provides a chemically defined culture system, by which induced pluripotent stem (iPS) cells are obtained with high efficiency. The culture medium supplement of the present invention includes vitamin C and a glycogen synthase kinase-3 inhibitor; another culture medium supplement of the present invention further includes, in addition to vitamin C and the glycogen synthase kinase-3 inhibitor, vitamin B 12, insulin, a receptor tyrosine kinase, and an anti-oxidant; and the culture medium supplement of the present invention may further be a mixture of the above two culture medium supplements with a serum replacement cell growth promoter. The present invention further provides a complete culture medium for iPS cells, which is formed by one or more of a basal culture medium, serum, and a serum replacement supplement, and the above culture medium supplements, or formed only by the above culture medium supplements and a basal culture medium. The culture system of the present invention is a chemically defined culture system free of serum and contamination from animal source, by which the iPS cells are obtained with high efficiency. The culture system maintains the growth and proliferation of the cells in conversion of somatic cells to iPS cells in absence of feeder cells, significantly accelerates the iPS cell induction process, and greatly improves the induction efficiency of somatic cells into iPS cells.

    摘要翻译： 本发明提供一种化学上定义的培养系统，通过该培养系统以高效率获得诱导的多能干细胞（iPS）细胞。 本发明的培养基补充剂包括维生素C和糖原合酶激酶-3抑制剂; 除了维生素C和糖原合成酶激酶-3抑制剂之外，本发明的另一种培养基补充剂还包括维生素B 12，胰岛素，受体酪氨酸激酶和抗氧化剂; 并且本发明的培养基补充物还可以是上述两种培养基补充物与血清替代细胞生长促进剂的混合物。 本发明还提供一种用于iPS细胞的完整培养基，其由一种或多种基础培养基，血清和血清替代补充剂形成，并且上述培养基补充剂或仅由上述培养基补充剂形成 和基础培养基。 本发明的培养体系是一种化学上定义的不含动物源血清和污染物的培养系统，通过该培养系统获得高效率的iPS细胞。 培养系统保持细胞在不存在饲养细胞时体细胞转化为iPS细胞的生长和增殖，显着加速iPS细胞诱导过程，并大大提高体细胞诱导iPS细胞的诱导效率。