- 专利标题： METHODS AND COMPOSITION TO GENERATE UNIQUE SEQUENCE DNA PROBES, LABELING OF DNA PROBES AND THE USE OF THESE PROBES
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申请号： EP17163260.7申请日： 2006-09-20
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公开(公告)号： EP3211086A3公开(公告)日： 2017-11-08
- 发明人: CONNELLY, Mark, Carle , FOULK, Brad , KAGAN, Michael, T. , SWENNENHUIS, Joost, F. , TERSTAPPEN, Leon, W.M.M. , TIBBE, Arjan, G.J. , VERRANT, John, A.
- 申请人： Janssen Diagnostics, LLC
- 申请人地址： 700 US Highway 202 Raritan, NJ 08869 US
- 专利权人： Janssen Diagnostics, LLC
- 当前专利权人： Janssen Diagnostics, LLC
- 当前专利权人地址： 700 US Highway 202 Raritan, NJ 08869 US
- 代理机构： Hanson, William Bennett
- 优先权： US718676P 20050920; US729536P 20051024; US786117P 20060327
- 主分类号： C12P19/34
- IPC分类号： C12P19/34 ; C07H21/04
摘要：
A method for detecting the presence of a target sequence comprising:
a. adding a probe, said probe having been produced by a method comprising:
i. obtaining double strand polynucleotides known to contain complementary target sequences and repetitive sequences;
ii. fragmenting said double strand polynucleotides into fragments;
iii. denaturing said fragments into single strands;
iv. hybridizing said repetitive sequences to form a mixture of double strands and single strands;
v. cleaving said double strands; and
vi. amplifying said single strands wherein said single strands are complementary to said target sequences; and
b. detecting said probe wherein said detection is selected from a group consisting of ISH, FISH, CGH, spectral karyotyping, chromosome painting, Northern blots, Southern blots, microarray analysis, and combinations thereof.
a. adding a probe, said probe having been produced by a method comprising:
i. obtaining double strand polynucleotides known to contain complementary target sequences and repetitive sequences;
ii. fragmenting said double strand polynucleotides into fragments;
iii. denaturing said fragments into single strands;
iv. hybridizing said repetitive sequences to form a mixture of double strands and single strands;
v. cleaving said double strands; and
vi. amplifying said single strands wherein said single strands are complementary to said target sequences; and
b. detecting said probe wherein said detection is selected from a group consisting of ISH, FISH, CGH, spectral karyotyping, chromosome painting, Northern blots, Southern blots, microarray analysis, and combinations thereof.
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