公开(公告)号：EP3211086A3

公开(公告)日：2017-11-08

申请号：EP17163260.7

申请日：2006-09-20

申请人： Janssen Diagnostics, LLC

发明人： CONNELLY, Mark, Carle ,  FOULK, Brad ,  KAGAN, Michael, T. ,  SWENNENHUIS, Joost, F. ,  TERSTAPPEN, Leon, W.M.M. ,  TIBBE, Arjan, G.J. ,  VERRANT, John, A.

IPC分类号： C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q1/34 ,  C12Q1/6811 ,  C12Q1/6876 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2533/101 ,  C12Q2531/113 ,  C12Q2521/301 ,  C12Q2563/131 ,  C12Q2563/103

摘要： A method for detecting the presence of a target sequence comprising:
a. adding a probe, said probe having been produced by a method comprising:
i. obtaining double strand polynucleotides known to contain complementary target sequences and repetitive sequences;
ii. fragmenting said double strand polynucleotides into fragments;
iii. denaturing said fragments into single strands;
iv. hybridizing said repetitive sequences to form a mixture of double strands and single strands;
v. cleaving said double strands; and
vi. amplifying said single strands wherein said single strands are complementary to said target sequences; and

b. detecting said probe wherein said detection is selected from a group consisting of ISH, FISH, CGH, spectral karyotyping, chromosome painting, Northern blots, Southern blots, microarray analysis, and combinations thereof.

公开(公告)号：EP1941050B1

公开(公告)日：2017-04-26

申请号：EP06844178.1

申请日：2006-09-20

申请人： Janssen Diagnostics, LLC

发明人： CONNELLY, Mark, Carle ,  FOULK, Brad ,  KAGAN, Michael, T. ,  SWENNENHUIS, Joost, F. ,  TERSTAPPEN, Leon, W.M.M. ,  TIBBE, Arjan, G.J. ,  VERRANT, John, A.

IPC分类号： C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q1/34 ,  C12Q1/6811 ,  C12Q1/6876 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2533/101 ,  C12Q2531/113 ,  C12Q2521/301 ,  C12Q2563/131 ,  C12Q2563/103

摘要： A method for detecting the presence of a target sequence comprising: a. adding a probe, said probe having been produced by a method comprising: i. obtaining double strand polynucleotides known to contain complementary target sequences and repetitive sequences; ii. fragmenting said double strand polynucleotides into fragments; iii. denaturing said fragments into single strands; iv. hybridizing said repetitive sequences to form a mixture of double strands and single strands; v. cleaving said double strands; and vi. amplifying said single strands wherein said single strands are complementary to said target sequences; and b. detecting said probe wherein said detection is selected from a group consisting of ISH, FISH, CGH, spectral karyotyping, chromosome painting, Northern blots, Southern blots, microarray analysis, and combinations thereof.

摘要翻译： 本发明涉及基因集会的DNA序列，基因或染色体识别领域。 被提供的方法和组合物以获得独特序列的DNA探针。 组合物包含任何双链DNA含有来自哪个重复序列被消除gemäß对本发明描述的方法的独特序列的。 因此，本发明涉及细胞免疫磁性选择和为了つ荧光标记后thathave被鉴定weiterer询问感兴趣的细胞的保存。 进一步，本发明涉及细胞免疫磁性选择和荧光标记后thathave被鉴定的遗传分析。

公开(公告)号：EP3211086A2

公开(公告)日：2017-08-30

申请号：EP17163260.7

申请日：2006-09-20

申请人： Janssen Diagnostics, LLC

发明人： CONNELLY, Mark, Carle ,  FOULK, Brad ,  KAGAN, Michael, T. ,  SWENNENHUIS, Joost, F. ,  TERSTAPPEN, Leon, W.M.M. ,  TIBBE, Arjan, G.J. ,  VERRANT, John, A.

IPC分类号： C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q1/34 ,  C12Q1/6811 ,  C12Q1/6876 ,  C12Q2600/106 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2533/101 ,  C12Q2531/113 ,  C12Q2521/301 ,  C12Q2563/131 ,  C12Q2563/103

摘要： A method for detecting the presence of a target sequence comprising:
a. adding a probe, said probe having been produced by a method comprising:
i. obtaining double strand polynucleotides known to contain complementary target sequences and repetitive sequences;
ii. fragmenting said double strand polynucleotides into fragments;
iii. denaturing said fragments into single strands;
iv. hybridizing said repetitive sequences to form a mixture of double strands and single strands;
v. cleaving said double strands; and
vi. amplifying said single strands wherein said single strands are complementary to said target sequences; and

b. detecting said probe wherein said detection is selected from a group consisting of ISH, FISH, CGH, spectral karyotyping, chromosome painting, Northern blots, Southern blots, microarray analysis, and combinations thereof.

摘要翻译： 一种检测靶序列存在的方法，包括：a。 添加探针，所述探针已通过包括以下的方法生产：i。 获得已知含有互补靶序列和重复序列的双链多核苷酸; II。 将所述双链多核苷酸片段化成片段; III。 将所述片段变性成单链; IV。 使所述重复序列杂交以形成双链和单链的混合物; v。切割所述双链; 和vi。 扩增所述单链，其中所述单链与所述靶序列互补; 和b。 检测所述探针，其中所述检测选自由ISH，FISH，CGH，光谱核型分析，染色体涂片，Northern印迹，Southern印迹，微阵列分析及其组合组成的组。