The present invention provides a method of producing a composition containing docosahexaenoic acid as a constituent fatty acid of glycerides, comprising hydrolyzing a feedstock oil containing glycerides comprising docosahexaenoic acid as a constituent fatty acid by action of a Candida cylindracea derived lipase and a partial glyceride lipase, thereby increacing the proportion of docosahexaenoic acid in glyceride fractions, wherein the lipase hydrolysis reaction is performed at a temperature no higher than 30°C for a period no longer than 15 hours.