摘要:
The present invention provides a method of producing a composition containing docosahexaenoic acid as a constituent fatty acid of glycerides, comprising hydrolyzing a feedstock oil containing glycerides comprising docosahexaenoic acid as a constituent fatty acid by action of a Candida cylindracea derived lipase and a partial glyceride lipase, thereby increacing the proportion of docosahexaenoic acid in glyceride fractions, wherein the lipase hydrolysis reaction is performed at a temperature no higher than 30°C for a period no longer than 15 hours.
摘要:
The present invention provides a process for concentrated PUFA oil, characterized in that alcoholysis reaction using lipase is carried out in the presence of a small amount of water and at least one compound as an additive selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide, and then separation is conducted to obtain a glyceride fraction.
摘要:
Alcoholysis of oils and fats containing EPA and DHA is performed by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of a reaction additive such as magnesium oxide; then the glyceride fraction is separated; alcoholysis of the glyceride fraction is performed by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of a reaction additive such as magnesium oxide; and EPA-enriched oil and DHA-enriched oil are simultaneously obtained.
摘要:
In the specification, a method for purifying a compound by column chromatography, and a method for optimizing the symmetry factor and theoretical plate number by allowing the slurry to settle and reducing the sedimentation volume of the slurry. A compound purified by such method is also provided.
摘要:
The disclosure aims to provide compositions comprising highly enriched PUFA or alkyl ester thereof while containing fatty acid esters of 3-MCPD at adequately low concentrations and to provide an efficient method for producing the composition. The proposed composition contains fatty acids or fatty acid alkyl esters as its major component, the composition containing highly unsaturated fatty acid or alkyl ester thereof, wherein the proportion of the highly unsaturated fatty acid in the constituent fatty acids of the composition is 50 area% or more and wherein the concentration of 3-MCPD, as found by analysing the composition using American Oil Chemists' Society official method Cd 29b-13 assay A, is less than 1.80 ppm.
摘要:
The present invention provides a method of producing a composition containing docosahexaenoic acid as a constituent fatty acid of glycerides, comprising hydrolyzing a feedstock oil containing glycerides comprising docosahexaenoic acid as a constituent fatty acid by action of a first lipase and a second lipase, thereby increasing the proportion of docosahexaenoic acid in glyceride fractions, wherein the first lipase is at least one lipase selected from the group consisting of a lipase derived from a microorganism of the genus Thermomyces, a lipase derived from a microorganism of the genus Pseudomonas, a lipase derived from a microorganism of the genus Burkholderia, and a lipase derived from a microorganism of the genus Alcaligenes, and wherein the second lipase is a partial glyceride lipase.
摘要:
The present invention provides a process for concentrated PUFA oil, characterized in that alcoholysis reaction using lipase is carried out in the presence of a small amount of water and at least one compound as an additive selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide, and then separation is conducted to obtain a glyceride fraction.