A biological particle analysis method (S100) is provided and includes the following steps: fluorescence staining a liquid specimen (200) through a fluorescence staining process so as to enable a target biological particle (201a) in the liquid specimen (200) to becomes a fluorescence; accommodating the liquid specimen (200) into a pico-droplet generator (21) and using a camera device (23) to take a real-time image of the liquid specimen (200); using the pico-droplet generator (21) to output a target pico-droplet (202a) having the target biological particle (201a) onto a biochip (22) according to the real-time image; removing the fluorescent color of the target biological particle (201a) in the target pico-droplet (202a) through a washing process; and fluorescence staining the target biological particle (201a) captured by the biochip (22) at multiple times through the fluorescence staining process and the washing process, so as to obtain a plurality of fluorescence images respectively corresponding to multiple kinds of biological characterization expressions.