公开(公告)号：EP4361591A1

公开(公告)日：2024-05-01

申请号：EP23168000.0

申请日：2023-04-14

申请人： Cytoaurora Biotechnologies, Inc.

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng ,  YE, Guang-Ci

IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N1/31 ,  B01L3/02 ,  B01L3/00 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N1/30 ,  G01N2015/001920130101 ,  G01N2015/100620130101 ,  G01N2015/141520130101 ,  G01N2015/14220130101 ,  G01N15/1404 ,  G01N2015/148120130101 ,  G01N15/1459 ,  G01N15/1425 ,  G01N15/1484 ,  G01N1/312 ,  B01L3/0268 ,  B01L3/5088 ,  B01L2400/08620130101 ,  G01N2015/01220240101 ,  G01N15/1433 ,  G01N15/1492

摘要： A biological particle analysis method (S100) is provided and includes the following steps: fluorescence staining a liquid specimen (200) through a fluorescence staining process so as to enable a target biological particle (201a) in the liquid specimen (200) to becomes a fluorescence; accommodating the liquid specimen (200) into a pico-droplet generator (21) and using a camera device (23) to take a real-time image of the liquid specimen (200); using the pico-droplet generator (21) to output a target pico-droplet (202a) having the target biological particle (201a) onto a biochip (22) according to the real-time image; removing the fluorescent color of the target biological particle (201a) in the target pico-droplet (202a) through a washing process; and fluorescence staining the target biological particle (201a) captured by the biochip (22) at multiple times through the fluorescence staining process and the washing process, so as to obtain a plurality of fluorescence images respectively corresponding to multiple kinds of biological characterization expressions.

公开(公告)号：EP4360759A1

公开(公告)日：2024-05-01

申请号：EP23168003.4

申请日：2023-04-14

申请人： Cytoaurora Biotechnologies, Inc.

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng ,  YE, Guang-Ci

IPC分类号： B01L3/02 ,  B01L3/00 ,  G01N1/30 ,  G01N15/14 ,  G01N1/31 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N1/30 ,  G01N2015/001920130101 ,  G01N2015/100620130101 ,  G01N2015/141520130101 ,  G01N2015/14220130101 ,  G01N15/1404 ,  G01N2015/148120130101 ,  G01N15/1459 ,  G01N15/1425 ,  G01N15/1484 ,  G01N1/312 ,  B01L3/0268 ,  B01L3/5088 ,  B01L2400/08620130101 ,  G01N2015/01220240101 ,  G01N15/1433 ,  G01N15/1492

摘要： A biological particle enrichment apparatus (2) and a pico-droplet generator (21) thereof are provided. The pico-droplet generator (21) includes a container (211), a hollow needle (212) connected to the container (211), a first piezoelectric member (213) disposed on the container (211), and a second piezoelectric member (214) disposed on the hollow needle (212). The container (211) can receive a liquid specimen (200) having biological particles (201). The hollow needle (212) and the container (211) are fluid communicated with each other, and an inner diameter (D211) of the container (211) is within a range from 5 times to 30 times of an inner diameter (D212) of the hollow needle (212). The first piezoelectric member (213) is annularly disposed on a surrounding lateral side (2112) of the container (211), and enables the biological particles (201) in the container (211) to move along a direction away from the surrounding lateral side (2112) by vibrating the container (211). The second piezoelectric member (214) can squeeze the hollow needle (212), so that the liquid specimen (200) flows outwardly to form a pico-droplet (202).

公开(公告)号：EP3789115B1

公开(公告)日：2023-03-22

申请号：EP20176754.8

申请日：2020-05-27

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng ,  JOU, Hei-Jen ,  CHEN, Ming

IPC分类号： B01L3/00