公开(公告)号：EP4321249A1

公开(公告)日：2024-02-14

申请号：EP22210197.4

申请日：2022-11-29

申请人： Cytoaurora Biotechnologies, Inc.

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng

IPC分类号： B01L3/00 ,  B03C5/00 ,  G01N15/14 ,  H01L31/0224 ,  H01L31/10 ,  B03C5/02 ,  H01L27/146 ,  H01L29/732

摘要： A contactless selection device (100), a light sensing structure (1) thereof, and a biological particle selection apparatus (1000) are provided. The light sensing structure (1) includes a substrate, an insulating layer (14), an electrode layer, and a photoelectric layer (13), the latter two of which are respectively formed on two opposite sides of the substrate. The photoelectric layer (13) includes a plurality of collector regions (1312), a plurality of base regions (1321) respectively formed in the collector regions (1312), and a plurality of emitter regions (1331) that are respectively formed in the base regions (1321). Each of the emitter regions (1331) includes a plurality of emitter pads (1332) formed in the corresponding base region (1321). Each of the base regions (1321), the corresponding collector region (1312), and the corresponding emitter region (1331) are jointly formed as a vertical transistor (130). The insulating layer (14) covers and separates the vertical transistors (130) and an end of each of the emitter pads (1332) is exposed from the insulating layer (14).

公开(公告)号：EP4360759A1

公开(公告)日：2024-05-01

申请号：EP23168003.4

申请日：2023-04-14

申请人： Cytoaurora Biotechnologies, Inc.

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng ,  YE, Guang-Ci

IPC分类号： B01L3/02 ,  B01L3/00 ,  G01N1/30 ,  G01N15/14 ,  G01N1/31 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N1/30 ,  G01N2015/001920130101 ,  G01N2015/100620130101 ,  G01N2015/141520130101 ,  G01N2015/14220130101 ,  G01N15/1404 ,  G01N2015/148120130101 ,  G01N15/1459 ,  G01N15/1425 ,  G01N15/1484 ,  G01N1/312 ,  B01L3/0268 ,  B01L3/5088 ,  B01L2400/08620130101 ,  G01N2015/01220240101 ,  G01N15/1433 ,  G01N15/1492

摘要： A biological particle enrichment apparatus (2) and a pico-droplet generator (21) thereof are provided. The pico-droplet generator (21) includes a container (211), a hollow needle (212) connected to the container (211), a first piezoelectric member (213) disposed on the container (211), and a second piezoelectric member (214) disposed on the hollow needle (212). The container (211) can receive a liquid specimen (200) having biological particles (201). The hollow needle (212) and the container (211) are fluid communicated with each other, and an inner diameter (D211) of the container (211) is within a range from 5 times to 30 times of an inner diameter (D212) of the hollow needle (212). The first piezoelectric member (213) is annularly disposed on a surrounding lateral side (2112) of the container (211), and enables the biological particles (201) in the container (211) to move along a direction away from the surrounding lateral side (2112) by vibrating the container (211). The second piezoelectric member (214) can squeeze the hollow needle (212), so that the liquid specimen (200) flows outwardly to form a pico-droplet (202).

公开(公告)号：EP4361591A1

公开(公告)日：2024-05-01

申请号：EP23168000.0

申请日：2023-04-14

申请人： Cytoaurora Biotechnologies, Inc.

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng ,  YE, Guang-Ci

IPC分类号： G01N1/30 ,  G01N15/14 ,  G01N1/31 ,  B01L3/02 ,  B01L3/00 ,  G01N15/00 ,  G01N15/10

CPC分类号： G01N1/30 ,  G01N2015/001920130101 ,  G01N2015/100620130101 ,  G01N2015/141520130101 ,  G01N2015/14220130101 ,  G01N15/1404 ,  G01N2015/148120130101 ,  G01N15/1459 ,  G01N15/1425 ,  G01N15/1484 ,  G01N1/312 ,  B01L3/0268 ,  B01L3/5088 ,  B01L2400/08620130101 ,  G01N2015/01220240101 ,  G01N15/1433 ,  G01N15/1492

摘要： A biological particle analysis method (S100) is provided and includes the following steps: fluorescence staining a liquid specimen (200) through a fluorescence staining process so as to enable a target biological particle (201a) in the liquid specimen (200) to becomes a fluorescence; accommodating the liquid specimen (200) into a pico-droplet generator (21) and using a camera device (23) to take a real-time image of the liquid specimen (200); using the pico-droplet generator (21) to output a target pico-droplet (202a) having the target biological particle (201a) onto a biochip (22) according to the real-time image; removing the fluorescent color of the target biological particle (201a) in the target pico-droplet (202a) through a washing process; and fluorescence staining the target biological particle (201a) captured by the biochip (22) at multiple times through the fluorescence staining process and the washing process, so as to obtain a plurality of fluorescence images respectively corresponding to multiple kinds of biological characterization expressions.

公开(公告)号：EP3789115A1

公开(公告)日：2021-03-10

申请号：EP20176754.8

申请日：2020-05-27

申请人： Cytoaurora Biotechnologies, Inc.

发明人： HUANG, Chung-Er ,  CHEN, Sheng-Wen ,  HO, Hsin-Cheng ,  JOU, Hei-Jen ,  CHEN, Ming

IPC分类号： B01L3/00

摘要： A catcher (C), a capture device (D), and a method for capturing at least one target biological particle (T) are provided. The catcher (C) includes a base (10) and a plurality of capture arms (20) extending from the base (10) and spaced apart from each other. Each of the capture arms (20) has a free end portion (21) configured to capture a target biological particle (T) and a supporting segment (22) connected between the free end portion (21) and the base (10). The supporting segment (22) of each of the capture arms (20) is arranged in a projection space defined by orthogonally projecting the free end portion (21) along a height direction (H) onto the base (10). When the target biological particle (T) is captured by two of the capture arms (20) that are bent and arranged adjacent to each other, a part of the target biological particle (T) is trapped by the supporting segments (22) of the two of the capture arms (20) and is hold.