PROBLEM TO BE SOLVED: To obtain the subject preparation capable of simple administration, effective start of the action, increasing half life and preventing adverse reaction of agglomeration by including specific components which are clotting factors. SOLUTION: The preparation contains at least two clotting factors which are the components of prothrombinase or pro-prothrombinase, not containing phospholipid. The preparation contains at least one of the factors activated, has at least two clotting factors selected from factors II, V, Va, X and Xa, practically factors II and X or Xa (capable of accompanying V or Va freely), the factor exists as a conjugate, preferably containing magnesium ion but free from free calcium ion.