An actuating structure of battery safety valve includes an end plate, a safety valve, a positive-terminal conductive plate, a negative-terminal conductive plate, a ventilate plate and an actuating plate. A positive terminal and a negative terminal penetrate through the end plate and the safety valve. The positive-terminal and the negative-terminal conductive plates are disposed on the end plate and extended from the positive terminal and the negative terminal to the safety valve, respectively. The ventilate plate is disposed on the end plate and corresponding to the safety valve. The actuating plate is disposed between the ventilate plate and the safety valve, and restricted under a normal condition by the safety valve. When an internal pressure of the battery exceeds a breaking pressure of the safety valve, the actuating plate is released, so that an electric connection path is formed between the positive and the negative terminals.