A driving method of a display device includes sequentially outputting a plurality of eye tuning signals, receiving a plurality of checking information obtained from a data driving circuit, wherein the checking information indicates whether the data driving circuit is operating in response to each of the plurality of eye tuning signals, and selecting one optimal eye tuning signal among the plurality of eye tuning signals operating the data driving circuit on the basis of the checking information. Image signals are output on the basis of condition information of the optimal eye tuning signal.