A light-emitting device and a method of manufacturing a light-emitting device are provided. The light-emitting device includes a transparent substrate having a first surface and a second surface opposite to the first surface, a light-emitting structure disposed on the first surface of the transparent substrate, a sealing layer, a carrier board, and a positive electrode and a negative electrode. The transparent substrate, the light-emitting structure, the sealing layer and the carrier board have corresponding through holes respectively, and at least one of the positive electrode and the negative electrode is disposed on the second surface of the transparent substrate.