发明授权
- 专利标题: Methods and devices based upon a novel form of nucleic acid duplex on a surface
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申请号: US12080720申请日: 2008-04-04
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公开(公告)号: US10272409B2公开(公告)日: 2019-04-30
- 发明人: Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
- 申请人: Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
- 代理机构: Ramey & Schwaller, LLP
- 代理商 William P. Ramey, III; Melissa D. Schwaller
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12P19/34 ; B01J19/00
摘要:
Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.