A positioning tag replacement method and a positioning server to implement replacement between positioning tags, where the method includes obtaining, by a positioning server, a moving trajectory of a first positioning tag and a moving trajectory of a second positioning tag, where the first positioning tag corresponds to a first positioned object, and the second positioning tag has no corresponding positioned object, and setting, by the positioning server, the second positioning tag to correspond to the first positioned object when the positioning server determines that the moving trajectory of the first positioning tag and the moving trajectory of the second positioning tag meet a preset condition. The method features low costs, simple and convenient operations, and easy implementation, and avoids that in an operation process, a positioned object escapes being monitored in a positioning system.