- 专利标题: Transgenic animal for production of antibodies having minimal CDRs
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申请号: US16209807申请日: 2018-12-04
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公开(公告)号: US10362770B2公开(公告)日: 2019-07-30
- 发明人: William Don Harriman , Robert Etches , Philip A. Leighton
- 申请人: Crystal Bioscience Inc.
- 申请人地址: US CA San Diego
- 专利权人: Crystal Bioscience Inc.
- 当前专利权人: Crystal Bioscience Inc.
- 当前专利权人地址: US CA San Diego
- 代理机构: Bozicevic, Field & Francis LLP
- 代理商 James S. Keddie
- 主分类号: A01K67/00
- IPC分类号: A01K67/00 ; A01K67/027 ; C12N15/85 ; C07K16/00 ; C12N15/87 ; C12P21/02
摘要:
A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.
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