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    Transgenic animal for production of antibodies having minimal CDRs
    1.
    发明授权
    Transgenic animal for production of antibodies having minimal CDRs 有权

    公开(公告)号：US10362770B2

    公开(公告)日：2019-07-30

    申请号：US16209807

    申请日：2018-12-04

    申请人： Crystal Bioscience Inc.

    发明人： William Don Harriman ,  Robert Etches ,  Philip A. Leighton

    IPC分类号： A01K67/00 ,  A01K67/027 ,  C12N15/85 ,  C07K16/00 ,  C12N15/87 ,  C12P21/02

    摘要： A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

    Transgenic animal for production of antibodies having minimal CDRs
    5.
    发明授权
    Transgenic animal for production of antibodies having minimal CDRs 有权

    公开(公告)号：US10687519B2

    公开(公告)日：2020-06-23

    申请号：US16428763

    申请日：2019-05-31

    申请人： Crystal Bioscience Inc.

    发明人： William Don Harriman ,  Robert Etches ,  Philip A. Leighton

    IPC分类号： A01K67/00 ,  A01K67/027 ,  C07K16/00 ,  C12N15/85 ,  C12N15/87 ,  C12P21/02

    摘要： A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

    TRANSGENIC ANIMAL FOR PRODUCTION OF ANTIBODIES HAVING MINIMAL CDRS
    7.
    发明申请
    TRANSGENIC ANIMAL FOR PRODUCTION OF ANTIBODIES HAVING MINIMAL CDRS 有权
    用于生产具有最小CDRs的抗体的转基因动物

    公开(公告)号：US20160304585A1

    公开(公告)日：2016-10-20

    申请号：US15188724

    申请日：2016-06-21

    申请人： Crystal Bioscience Inc.

    发明人： William Don Harriman ,  Robert Etches ,  Phil Leighton

    IPC分类号： C07K16/00 ,  C12N15/85 ,  A01K67/027

    CPC分类号： A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/567 ,  C12N15/8509 ,  C12N15/87 ,  C12N2015/8518 ,  C12N2517/02 ,  C12P21/02

    摘要： A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

    摘要翻译： 提供转基因动物。 在某些实施方案中，转基因动物包含基因组，其包含：免疫球蛋白轻链基因座，其包含：a）功能性免疫球蛋白轻链基因，其包含编码以下的转录可变区： 由2至5个不同氨基酸组成的轻链CDR1，CDR2和CDR3区; 和ii。 轻链框架; 并且可操作地连接到功能性免疫球蛋白轻链基因：b）多个伪基因轻链可变区，每个编码如下： 由与功能基因的CDR相同的2至5个不同氨基酸组成的轻链CDR1，CDR2和CDR3区; 和ii。 氨基酸序列与转录可变区的轻链骨架相同的轻链框架。

    Transgenic animal for production of antibodies having minimal CDRs
    9.
    发明授权
    Transgenic animal for production of antibodies having minimal CDRs 有权
    用于产生具有最小CDRs的抗体的转基因动物

    公开(公告)号：US09404125B2

    公开(公告)日：2016-08-02

    申请号：US14057820

    申请日：2013-10-18

    申请人： Crystal Bioscience Inc.

    发明人： William Don Harriman ,  Robert Etches ,  Phil Leighton

    IPC分类号： C12N15/85 ,  A01K67/027 ,  C07K16/00

    CPC分类号： A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/565 ,  C07K2317/567 ,  C12N15/8509 ,  C12N15/87 ,  C12N2015/8518 ,  C12N2517/02 ,  C12P21/02

    摘要： A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids; and ii. a light chain framework; and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.

    摘要翻译： 提供转基因动物。 在某些实施方案中，转基因动物包含基因组，其包含：免疫球蛋白轻链基因座，其包含：a）功能性免疫球蛋白轻链基因，其包含编码以下的转录可变区： 由2至5个不同氨基酸组成的轻链CDR1，CDR2和CDR3区; 和ii。 轻链框架; 并且可操作地连接到功能性免疫球蛋白轻链基因：b）多个伪基因轻链可变区，每个编码如下： 由与功能基因的CDR相同的2至5个不同氨基酸组成的轻链CDR1，CDR2和CDR3区; 和ii。 氨基酸序列与转录可变区的轻链骨架相同的轻链框架。

    Transgenic animal for producing diversified antibodies that have the same light chain I
    10.
    发明授权
    Transgenic animal for producing diversified antibodies that have the same light chain I 有权

    公开(公告)号：US11337409B2

    公开(公告)日：2022-05-24

    申请号：US15734187

    申请日：2019-06-05

    申请人： Crystal Bioscience Inc.

    发明人： Philip A. Leighton ,  William Don Harriman ,  Robert Etches

    IPC分类号： A01K67/027 ,  C07K16/00 ,  C12N15/02 ,  C12N15/85 ,  C12N5/0781

    摘要： This disclosure provides, among other things, strategies for minimizing antibody diversification in a transgenic animal that uses gene conversion for antibody diversification. In some embodiments, the animal may comprise a genome comprising an endogenous immunoglobulin light chain locus comprising: (a) a functional immunoglobulin light chain gene comprising a nucleic acid encoding a light chain variable region; and (b) a plurality of pseudogenes that are operably linked to the functional immunoglobulin light chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding a light chain variable region, wherein the pseudogenes are upstream or downstream of the functional immunoglobulin light chain gene and encode the same amino acid sequence as the light chain variable region of the functional immunoglobulin light chain gene of (a). In other embodiments, the locus may have a tandem array of coding sequences for the light chain.