Invention Grant
- Patent Title: Method for producing amniotic mesenchymal stromal cell composition, method for cryopreserving the same, and therapeutic agent
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Application No.: US14912662Application Date: 2014-08-18
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Publication No.: US10441611B2Publication Date: 2019-10-15
- Inventor: Kenichi Yamahara , Akihiko Taguchi , Toshihiro Soma , Shunsuke Ohnishi , Akira Kobayashi
- Applicant: NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER , FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE , HYOGO COLLEGE OF MEDICINE , NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY , KANEKA CORPORATION
- Applicant Address: JP Suita-shi JP Kobe-shi JP Nishinomiya-shi JP Sapporo-shi JP Osaka-shi
- Assignee: NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER,FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE,HYOGO COLLEGE OF MEDICINE,NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY,KANEKA CORPORATION
- Current Assignee: NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER,FOUNDATION FOR BIOMEDICAL RESEARCH AND INNOVATION AT KOBE,HYOGO COLLEGE OF MEDICINE,NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY,KANEKA CORPORATION
- Current Assignee Address: JP Suita-shi JP Kobe-shi JP Nishinomiya-shi JP Sapporo-shi JP Osaka-shi
- Agency: Oblon, McClelland Maier & Neustadt, L.L.P.
- Priority: JP2013-170008 20130819; JP2014-130142 20140625
- International Application: PCT/JP2014/071546 WO 20140818
- International Announcement: WO2015/025810 WO 20150226
- Main IPC: A61K35/50
- IPC: A61K35/50 ; A61K35/28 ; C12N5/0775 ; A61K9/00 ; A61L27/38 ; C12N5/073 ; C12N5/077 ; A61L27/50
Abstract:
An object of the present invention is to provide a method for producing a mesenchymal stromal cell composition, comprising conveniently and aseptically separating high-purity amnion-derived MSCs by performing enzyme treatment only once. According to the present invention, the following are provided: a method for producing a mesenchymal stromal cell composition, comprising: performing enzyme treatment of an amnion with collagenase and thermolysin and/or dispase; and filtering the enzyme-treated amnion through a mesh; a method for producing a cryopreserved mesenchymal stromal cell composition; and a therapeutic agent comprising as an active ingredient the mesenchymal stromal cell composition for a disease selected from graft-versus-host disease, inflammatory bowel disease, systemic lupus erythematosus, liver cirrhosis, or radiation enteritis.
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