Invention Grant
- Patent Title: Phospholipase C
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Application No.: US15564275Application Date: 2016-04-08
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Publication No.: US10457890B2Publication Date: 2019-10-29
- Inventor: Arjen Sein , Rene Marcel De Jong , Evert Tjeerd Van Rij , Willem Bijleveld , Maarten Hotse Wilbrink
- Applicant: DSM IP ASSETS B.V.
- Applicant Address: NL Heerlen
- Assignee: DSM IP ASSETS B.V.
- Current Assignee: DSM IP ASSETS B.V.
- Current Assignee Address: NL Heerlen
- Agency: McBee Moore Woodward & Vanik IP, LLC
- Priority: EP15162972 20150409
- International Application: PCT/EP2016/057690 WO 20160408
- International Announcement: WO2016/162456 WO 20161013
- Main IPC: C11B3/00
- IPC: C11B3/00 ; C12N9/16 ; C12P7/64 ; C12P7/20

Abstract:
The present invention relates to a polypeptide having phospholipase C activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. A process for degumming a vegetable oil comprising contacting a vegetable oil comprising phospholipids with a polypeptide having phospholipase C activity of the invention or a composition of the invention, wherein phospholipids are hydrolyzed into diacylglycerol and phosphate ester and/or phosphate, separating the phosphate ester and/or phosphate from the vegetable oil wherein a degummed vegetable oil is obtained.
Public/Granted literature
- US20180134986A1 PHOSPHOLIPASE C Public/Granted day:2018-05-17
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