公开(公告)号：US11667902B2

公开(公告)日：2023-06-06

申请号：US17109759

申请日：2020-12-02

Applicant: DSM IP ASSETS B.V.

Inventor： Rene Marcel De Jong ,  Chantal Christis ,  Neil Carr

IPC: C12N9/20 ,  A21D8/04

CPC classification number: C12N9/20 ,  A21D8/042 ,  C12Y301/01003

Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US10167463B2

公开(公告)日：2019-01-01

申请号：US14397865

申请日：2013-05-03

Applicant: DSM IP ASSETS B.V.

Inventor： Petrus Jacobus Theodorus Dekker ,  Rene Marcel De Jong ,  Michael Dennis Tabeling ,  Cornelis Marinus Muijlwijk

IPC: C12N9/64 ,  A23C19/032 ,  A23C19/068 ,  A23C19/072

Abstract: The invention relates to a polypeptide having chymosin activity which: a. is capable of hydrolyzing bovine alpha s1-casein at position F23F24 so as to form αs1-I CN (f24-199) more rapidly than camel chymosin; and b. has a C/P ratio higher than the C/P ratio of bovine chymosin.

公开(公告)号：US20180030427A1

公开(公告)日：2018-02-01

申请号：US15782528

申请日：2017-10-12

Applicant: DSM IP ASSETS B.V.

Inventor： Petrus Jacobus Theodorus DEKKER ,  Rene Marcel De Jong ,  Cornelis Marinus Muijlwijk

IPC: C12N9/64 ,  A23C19/072 ,  A23C19/032 ,  A23C19/068

CPC classification number: C12N9/6483 ,  A23C19/032 ,  A23C19/0326 ,  A23C19/0684 ,  A23C19/072 ,  A23C2220/202 ,  A23C2250/15 ,  C12Y304/23004

Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.

公开(公告)号：US10655115B2

公开(公告)日：2020-05-19

申请号：US16250860

申请日：2019-01-17

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter Los ,  Rene Marcel De Jong ,  Maaike Appeldoorn

IPC: C12N9/24 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14

Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

公开(公告)号：US10000537B2

公开(公告)日：2018-06-19

申请号：US14895742

申请日：2014-06-04

Applicant: DSM IP ASSETS B.V.

Inventor： Paul Klaassen ,  Paulus Petrus De Waal ,  Rene Marcel De Jong ,  Arnold Jacob Mattieu Driessen ,  Jeroen Gerben Nijland ,  Hyun Yong Shin

IPC: C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C12N1/00 ,  C07H21/04 ,  C07K1/00 ,  C07K14/395 ,  C12P19/02

CPC classification number: C07K14/395 ,  C12P19/02

Abstract: The invention relates to a polypeptide having one or more substitution at a position corresponding to position 339 or 376 of SEQ ID NO: 59, wherein the polypeptide is a member of the Major Facilitator Superfamily (MFS). In an embodiment, the substitution is at position corresponding to 376 and wherein the amino acid at that position is replaced by an amino acid that has a van der Waals volume of 80 to 138 Å3 and a side chain hydrophobicity of 10 to 100 ΔtR.

公开(公告)号：US09822352B2

公开(公告)日：2017-11-21

申请号：US14397866

申请日：2013-05-03

Applicant: DSM IP ASSETS B.V.

Inventor： Petrus Jacobus Theodorus Dekker ,  Rene Marcel De Jong ,  Cornelis Marinus Muijlwijk

IPC: C12N9/64 ,  A23C19/032 ,  A23C19/068 ,  A23C19/072

CPC classification number: C12N9/6483 ,  A23C19/032 ,  A23C19/0326 ,  A23C19/0684 ,  A23C19/072 ,  A23C2220/202 ,  A23C2250/15 ,  C12Y304/23004

Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.

公开(公告)号：US10889807B2

公开(公告)日：2021-01-12

申请号：US16471121

申请日：2017-12-19

Applicant: DSM IP Assets B.V.

Inventor： Rene Marcel De Jong ,  Chantal Christis ,  Neil Carr

IPC: C12N15/00 ,  C12N9/16 ,  C12N9/18 ,  A21D8/04 ,  C12N9/20

Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295,
said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2.
Such a variant polypeptide may be used in the preparation of a baked product.

公开(公告)号：US20190136213A1

公开(公告)日：2019-05-09

申请号：US16250860

申请日：2019-01-17

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter LOS ,  Rene Marcel De Jong ,  Maaike Appeldoorn

IPC: C12N9/24 ,  C12P19/14 ,  C12P19/02 ,  C12P19/12

Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

公开(公告)号：US20170268012A1

公开(公告)日：2017-09-21

申请号：US15612728

申请日：2017-06-02

Applicant: DSM IP ASSETS B.V.

Inventor： Paul KLAASSEN ,  Gijsberdina Pieternella Van Suylekom ,  Rene Marcel De Jong

IPC: C12N15/81 ,  C12P5/02 ,  C12P7/10 ,  C12P7/56 ,  C12P7/54 ,  C12P7/48 ,  C12P7/46 ,  C12P7/42 ,  C12P35/00 ,  C12P7/20 ,  C12P7/18 ,  C12P7/16 ,  C12P7/06 ,  C12P7/40 ,  C12N9/92

CPC classification number: C12N15/81 ,  C12N9/92 ,  C12N15/815 ,  C12P5/026 ,  C12P7/06 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P35/00 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17

Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least 75% sequence identity to the amino acid sequence set out in SEQ ID NO: 2 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

公开(公告)号：US11447759B2

公开(公告)日：2022-09-20

申请号：US16788868

申请日：2020-02-12

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter Los ,  Rene Marcel De Jong ,  Maaike Appeldoorn

IPC: C12N9/24 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14

Abstract: The invention relates to a polypeptide having hemicellulase activity which comprises the amino acid sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66 or 71, SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69 or 74, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72 or the variant polynucleotide encodes a polypeptide that has at least 75% sequence identity with the sequence set out in SEQ ID NO: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67 or 72. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.