Invention Grant
- Patent Title: Method for increasing glycosylation of a composition comprising steviol glycosides
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Application No.: US15516854Application Date: 2015-10-08
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Publication No.: US10472661B2Publication Date: 2019-11-12
- Inventor: Marco Alexander Van Den Berg , Marcella Katharina Madern , Remko Tsjibbe Winter
- Applicant: DSM IP ASSETS B.V.
- Applicant Address: NL Heerlen
- Assignee: DSM IP ASSETS B.V.
- Current Assignee: DSM IP ASSETS B.V.
- Current Assignee Address: NL Heerlen
- Agency: McBee Moore Woodward & Vanik IP, LLC
- Priority: EP14188182 20141008
- International Application: PCT/EP2015/073281 WO 20151008
- International Announcement: WO2016/055578 WO 20160414
- Main IPC: C12P19/56
- IPC: C12P19/56 ; C12N15/52 ; A23L27/30 ; C12P19/18

Abstract:
The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises: a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.
Public/Granted literature
- US20170306377A1 STEVIOL GLYCOSIDE PRODUCTION Public/Granted day:2017-10-26
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