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公开(公告)号：US20240352501A1
公开(公告)日：2024-10-24
申请号：US18757644
申请日：2024-06-28
申请人： The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization
发明人： Maxim ITKIN , Rachel DAVIDOVICH-RIKANATI , Shahar COHEN , Vitaly PORTNOY , Adi DORON-FAIGENBOIM , Marina PETREIKOV , Shmuel SHEN , Yaakov TADMOR , Yosef BURGER , Efraim LEWINSOHN , Nurit KATZIR , Arthur Aaron SCHAFFER , Elad OREN
IPC分类号： C12P33/12 , A23L2/60 , A23L27/30 , C07J17/00 , C12N9/02 , C12N9/14 , C12N9/88 , C12N9/90 , C12P19/56 , C12P33/20
CPC分类号： C12P33/12 , A23L2/60 , A23L27/36 , C07J17/005 , C12N9/0071 , C12N9/0083 , C12N9/14 , C12N9/88 , C12N9/90 , C12P19/56 , C12P33/20 , C12Y114/99007 , C12Y204/02017 , C12Y402/01 , C12Y504/99033 , A23V2002/00
摘要： Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.
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公开(公告)号：US12084688B2
公开(公告)日：2024-09-10
申请号：US17470085
申请日：2021-09-09
申请人： RIKEN
发明人： Toshiya Muranaka , Hikaru Seki , Kazuki Saito , Kiyoshi Ohyama
IPC分类号： C12N9/10 , C12P19/18 , C12P19/56 , C12Q1/6895
CPC分类号： C12N9/1051 , C12P19/18 , C12P19/56 , C12Q1/6895 , C12Y204/01
摘要： Provided are an enzyme involved in a glycyrrhizin biosynthetic system, a gene of the enzyme and use thereof in order to stably and continuously provide a large amount of glycyrrhizin. Glucuronosyltransferase with an activity of further transferring glucuronic acid to the hydroxy group at the 2-position of glucuronic acid in an oleanane-type triterpenoid monoglucuronide is identified to provide the transferase, a gene for the transferase and use thereof.
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公开(公告)号：US12071646B2
公开(公告)日：2024-08-27
申请号：US17563127
申请日：2021-12-28
发明人： Maxim Itkin , Rachel Davidovich-Rikanati , Shahar Cohen , Vitaly Portnoy , Adi Doron-Faigenboim , Marina Petreikov , Shmuel Shen , Yaakov Tadmor , Yosef Burger , Efraim Lewinsohn , Nurit Katzir , Arthur A. Schaffer , Elad Oren
IPC分类号： C12N9/10 , A23L2/60 , A23L27/30 , C07J17/00 , C12N9/02 , C12N9/14 , C12N9/88 , C12N9/90 , C12P19/56 , C12P33/12 , C12P33/20
CPC分类号： C12P33/12 , A23L2/60 , A23L27/36 , C07J17/005 , C12N9/0071 , C12N9/0083 , C12N9/14 , C12N9/88 , C12N9/90 , C12P19/56 , C12P33/20 , C12Y114/99007 , C12Y204/02017 , C12Y402/01 , C12Y504/99033 , A23V2002/00
摘要： Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.
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公开(公告)号：US12065685B1
公开(公告)日：2024-08-20
申请号：US18368369
申请日：2023-09-14
申请人： AMYRIS, INC.
发明人： Sean Lund , Gale Wichmann
CPC分类号： C12P19/56 , C12N15/81 , C12Y106/02004 , C12Y114/14 , C12Y205/01029 , C12Y402/03019 , C12Y505/01012
摘要： Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of UGT76G1. The host cell further contains one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of rebaudioside M.
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公开(公告)号：US20240247296A1
公开(公告)日：2024-07-25
申请号：US18378023
申请日：2023-10-09
发明人： John Hart , Daniel Derkacs , Zachary Bittner , Andrew Espenlaub
IPC分类号： C12P19/56
CPC分类号： C12P19/56
摘要： A multijunction solar cell including an upper first solar subcell having a first band gap and positioned for receiving an incoming light beam; and a second solar subcell disposed below and adjacent to and lattice matched with said upper first solar subcell, and having a second band gap smaller than said first band gap; wherein at least one of the solar subcells has a graded band gap throughout the thickness of at least a portion of its emitter layer and base layer.
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公开(公告)号：US12012628B2
公开(公告)日：2024-06-18
申请号：US17105781
申请日：2020-11-27
申请人： Jiangnan University
发明人： Yongmei Xia , Zhuoyu Zhou , Ye Fan , Xueyi Hu , Yun Fang , Xiang Liu , Haijun Wang , Jing Wu , Fei Xu
CPC分类号： C12P19/56 , A23L27/36 , C12P19/14 , A23V2002/00
摘要： The disclosure discloses a method to elevate the amount of steviol glycosides remained in the recrystallization mother liquor of Stevia extract, and belongs to the field of modification and extraction of natural compounds. The disclosure adopts lactase for highly specific catalytic hydrolysis of stevioside to convert stevioside into rubusoside which usually can only be obtained from leaves of Rubus suavissimus S. Lee; and meanwhile, after stevioside in the mother liquor glycosides is converted, since the polarity difference between rubusoside and rebaudioside A is far larger than the polarity difference between stevioside and rebaudioside A, rebaudioside A is more easily separated out of the mother liquor glycosides. A technology provided by the disclosure is also applicable to value increment of mixed steviol glycosides containing stevioside and is beneficial for separating out rebaudioside A from the mixed steviol glycosides.
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公开(公告)号：US11952604B2
公开(公告)日：2024-04-09
申请号：US17805626
申请日：2022-06-06
申请人： PepsiCo, Inc.
发明人： Alex Tao , Guoqing Li , Wenxia Wang , Leilei Zheng , Chunlei Zhu , Xiaoliang Liang , Kuikiu Chan
CPC分类号： C12P19/56 , C12N9/1051 , C12Y204/01017
摘要： Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J.
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公开(公告)号：US11919920B2
公开(公告)日：2024-03-05
申请号：US17252266
申请日：2019-09-25
申请人： Firmenich SA
发明人： Xian-Wen Gan
摘要： The present disclosure relates generally to terpene glycosides, such as certain such compounds extracted from Stevia rebaudiana bertoni, Rubus suavissimus, or Siraitia grosvenorii. The disclosure also provides for the use of such compounds as food ingredients, flavors, and sweeteners, and related methods. The disclosure also provides ingestible compositions comprising such compounds, as well as processes for extracting such compounds selectively from certain plant sources.
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公开(公告)号：US20230413865A1
公开(公告)日：2023-12-28
申请号：US18155002
申请日：2023-01-16
申请人： PURECIRCLE SDN BHD
发明人： Avetik MARKOSYAN , Indra PRAKASH , Cyrille JARRIN , Aurélien BADIE , Robert TER HALLE , Cynthia CURRAN , Daniel AURIOL
CPC分类号： A23L2/60 , A23L27/36 , C07H1/06 , C07H15/256 , C12P19/18 , C12P19/56 , C12Y204/01 , C12Y302/01021 , Y02P20/582 , A23V2002/00
摘要： Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and Mare described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2, reb M2, and reb I are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.
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公开(公告)号：US11819042B2
公开(公告)日：2023-11-21
申请号：US16862214
申请日：2020-04-29
申请人： Manus Bio, Inc.
发明人： Ryan Philippe , Ajikumar Parayil Kumaran , Jason Donald , Krishna Patel , Swati Gupta , Ryan Lim , Liwei Li
CPC分类号： A23L2/60 , A23L27/36 , C12N9/0073 , C12N9/1048 , C12N9/1051 , C12P19/56 , C12Y114/13079 , C12Y204/01017 , A23V2002/00
摘要： The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.
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