公开(公告)号：US10472661B2

公开(公告)日：2019-11-12

申请号：US15516854

申请日：2015-10-08

Applicant: DSM IP ASSETS B.V.

Inventor： Marco Alexander Van Den Berg ,  Marcella Katharina Madern ,  Remko Tsjibbe Winter

IPC: C12P19/56 ,  C12N15/52 ,  A23L27/30 ,  C12P19/18

Abstract: The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises: a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.

公开(公告)号：US10787649B2

公开(公告)日：2020-09-29

申请号：US16316988

申请日：2017-07-11

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter Los ,  Rene Marcel De Jong ,  Remko Tsjibbe Winter ,  Ben Den Dulk

IPC: C12N9/04 ,  C12P7/46 ,  C12N1/20 ,  C12N15/00 ,  C12N1/14 ,  C12R1/865

Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.

公开(公告)号：US11339379B2

公开(公告)日：2022-05-24

申请号：US16999962

申请日：2020-08-21

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter Los ,  Rene Marcel De Jong ,  Remko Tsjibbe Winter ,  Ben Den Dulk

IPC: C12N9/04 ,  C12P7/46 ,  C12N1/20 ,  C12N15/00 ,  C12N1/14 ,  C12N1/18 ,  C12R1/865

Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.

公开(公告)号：US09944905B2

公开(公告)日：2018-04-17

申请号：US15103237

申请日：2014-12-12

Applicant: DSM IP ASSETS B.V.

Inventor： René Marcel De Jong ,  Zheng Zhao ,  Ben Den Dulk ,  Remko Tsjibbe Winter

IPC: C12P7/62 ,  C12P7/46 ,  C12N9/02 ,  C12P19/34 ,  C12P21/06 ,  C12N15/00 ,  C12N1/00 ,  C07H21/04 ,  C07K1/00

CPC classification number: C12N9/001 ,  C12P7/46 ,  C12Y103/01006

Abstract: The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.