Embodiments of an access point (AP), station (STA) and method for multi-user (MU) location measurement are generally described herein. The AP may contend for a transmission opportunity (TXOP) to obtain access to a channel. The AP may transmit a trigger frame (TF) to initiate a multi-user (MU) location measurement during the TXOP. The AP may receive service requests for the MU location measurement from a plurality of STAs. The AP may transmit an MU acknowledgement (ACK) frame that indicates reception of the service requests. The AP may receive, from the STAs, uplink sounding frames that include per-STA timing information for the service requests and the MU ACK frame. The STA may determine location measurements for the STAs based on the per-STA timing information included in the uplink sounding frames.