A cyclonic separating device for an upright vacuum cleaner and an upright vacuum cleaner with the same are disclosed. The cyclonic separating device includes a dirt cup, a filter and a cyclonic cone assembly. The filter is disposed in the dirt cup and includes a filter inlet and a filter outlet communicating between the filter inlet and an air outlet. The cyclonic cone assembly includes a cyclone cylinder and a cyclonic guide, in which a first end of the cyclone cylinder is communicated with an air inlet, and the cyclonic guide is fitted in a second end of the cyclone cylinder and is detachably fitted over the filter to spirally guide dirty air introduced into the cyclone cylinder from the air inlet to the filter inlet.