Invention Grant
- Patent Title: Methods, compositions and kits for small RNA capture, detection and quantification
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Application No.: US15066139Application Date: 2016-03-10
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Publication No.: US10563250B2Publication Date: 2020-02-18
- Inventor: Chunmei Liu , Shoulian Dong , Linda Wong
- Applicant: LIFE TECHNOLOGIES CORPORATION
- Applicant Address: US CA Carlsbad
- Assignee: Life Technologies Corporation
- Current Assignee: Life Technologies Corporation
- Current Assignee Address: US CA Carlsbad
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/686

Abstract:
Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small RNA by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.
Public/Granted literature
- US20160265031A1 METHODS, COMPOSITIONS AND KITS FOR SMALL RNA CAPTURE, DETECTION AND QUANTIFICATION Public/Granted day:2016-09-15
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