公开(公告)号：US11473132B2

公开(公告)日：2022-10-18

申请号：US16948442

申请日：2020-09-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6848

Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.

公开(公告)号：US10465237B2

公开(公告)日：2019-11-05

申请号：US15113030

申请日：2015-01-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carmen Gjerstad ,  Shoulian Dong

IPC: C12Q1/68 ,  C12Q1/6851 ,  G16B20/00 ,  G16B25/20

Abstract: A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. The method further includes determining an efficiency from the received amplification data from amplification of the first and second reference sample. The method includes determining a relative PCR efficiency for the biological sample. Next, the method includes determining a quantity of biological material in the biological sample using the relative PCR efficiency.

公开(公告)号：US09920360B2

公开(公告)日：2018-03-20

申请号：US14233711

申请日：2013-11-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Wong ,  Caifu Chen ,  Yalei Wu ,  Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

公开(公告)号：US11274340B2

公开(公告)日：2022-03-15

申请号：US16741471

申请日：2020-01-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chunmei Liu ,  Shoulian Dong ,  Linda Wong

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6851

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small RNA by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.

公开(公告)号：US20170022552A1

公开(公告)日：2017-01-26

申请号：US15113030

申请日：2015-01-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carmen Gjerstad ,  Shoulian Dong

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  G16B20/00 ,  G16B25/20 ,  C12Q2523/125 ,  C12Q2537/165 ,  C12Q2545/113 ,  C12Q2545/114

Abstract: A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. The method further includes determining an efficiency from the received amplification data from amplification of the first and second reference sample. The method includes determining a relative PCR efficiency for the biological sample. Next, the method includes determining a quantity of biological material in the biological sample using the relative PCR efficiency.

Abstract translation: 提供了一种用于定量生物样品中生物材料的方法。 该方法包括从第一和第二参考样品的扩增接收放大数据，并从生物样品的扩增接收扩增数据。 所述方法还包括根据来自第一和第二参考样本的放大的接收到的放大数据确定效率。 该方法包括确定生物样品的相对PCR效率。 接下来，该方法包括使用相对PCR效率确定生物样品中的生物材料的量。

公开(公告)号：US20140274774A1

公开(公告)日：2014-09-18

申请号：US14206995

申请日：2014-03-12

Applicant: Life Technologies Corporation

Inventor： Kelly Li ,  Shoulian Dong ,  Caifu Chen

IPC: C12Q1/68

CPC classification number: C12Q1/6858 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2565/1015

Abstract: The present disclosure is drawn to methods for detection, quantitation and analysis of nucleotides of interest, for example SNPs, in nucleic acid sequences of interest using universal FRET-based reporter primers.

Abstract translation: 本公开内容涉及使用通用的基于FRET的报道引物在感兴趣的核酸序列中检测，定量和分析目标核苷酸（例如SNP）的方法。

公开(公告)号：US10563250B2

公开(公告)日：2020-02-18

申请号：US15066139

申请日：2016-03-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chunmei Liu ,  Shoulian Dong ,  Linda Wong

IPC: C12P19/34 ,  C12Q1/686

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small RNA by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.

公开(公告)号：US20150105275A1

公开(公告)日：2015-04-16

申请号：US14233711

申请日：2013-11-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Wong ,  Caifu Chen ,  Yalei Wu ,  Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/68

CPC classification number: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6832 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2525/191 ,  C12Q2525/301

Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small RNAs, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3′ end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA ligation product.

Abstract translation: 本文提供了用于捕获，检测和定量成熟小RNA的方法，组合物和试剂盒。 方法的实施方案包括在5'和3'半简并连接夹板的存在下分别将5'和3'连接衔接子连接到成熟小RNA的5'和3'末端以产生连接产物。 其他实施方案包括具有通用逆转录引物的逆转录聚腺苷酸化成熟小RNA，并且在半退化连接夹板存在下将衔接子连接到cDNA的3'末端以产生cDNA连接产物。

公开(公告)号：US20230052754A1

公开(公告)日：2023-02-16

申请号：US17961419

申请日：2022-10-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shoulian Dong ,  Chunmei Liu

IPC: C12Q1/6855 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q1/6853

Abstract: The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.

公开(公告)号：US11254977B2

公开(公告)日：2022-02-22

申请号：US14206995

申请日：2014-03-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelly Li ,  Shoulian Dong ,  Caifu Chen

IPC: C12Q1/68 ,  C12Q1/6858

Abstract: The present disclosure is drawn to methods for detection, quantitation and analysis of nucleotides of interest, for example SNPs, in nucleic acid sequences of interest using universal FRET-based reporter primers.