- 专利标题: Methods and reagents for reverse-transcription polymerase chain reaction
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申请号: US15534578申请日: 2015-12-18
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公开(公告)号: US10858694B2公开(公告)日: 2020-12-08
- 发明人: Alexander Yarunin , Peter James Tatnell , Kathryn Louise Lamerton , Rebecca Fullerton
- 申请人: Global Life Sciences Solutions Operations UK Ltd
- 申请人地址: GB Sheffield
- 专利权人: Global Life Sciences Solutions Operations UK Ltd
- 当前专利权人: Global Life Sciences Solutions Operations UK Ltd
- 当前专利权人地址: GB Sheffield
- 代理机构: Eversheds Sutherland (US) LLP
- 优先权: GB1423080.9 20141223
- 国际申请: PCT/US2015/066584 WO 20151218
- 国际公布: WO2016/106113 WO 20160630
- 主分类号: C12Q1/6844
- IPC分类号: C12Q1/6844 ; C12Q1/6806
摘要:
The present invention provides a method of amplifying an RNA molecule in a biological sample by reverse transcription PCR (RT-PCR), wherein the RT-PCR is carried out in a solution comprising a) a polar aprotic solvent; b) a serum albumin; and optionally c) a non-ionic surfactant and/or a betaine.
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