Presented are a structured illumination microscopy system using a digital micromirror device and a time-complex structured illumination, and an operation method therefor. A structured illumination microscopy system using a digital micromirror device and a time-complex structured illumination according to an embodiment may comprise: a light source; a digital micromirror device (DMD) for receiving light irradiated from the light source, implementing a time-complex structured illumination, and causing a controlled structured illumination to enter a sample; and a fluorescence image measurement unit for extracting a high-resolution 3D fluorescence image of the sample.