A method and an apparatus of compensating a display device, and the display device are provided. The display device includes pixel units in multiple rows and multiple columns, each of the pixel units includes sub-pixels having different colors, and each of the sub-pixels includes a pixel driving circuit and a light-emitting element connected to the pixel driving circuit, the pixel driving circuit includes a sensing line for sensing an electrical signal from the light-emitting element. The method includes: sensing electrical signals from light-emitting elements of sub-pixels having at least two different colors by using sensing lines corresponding to the sub-pixels having the at least two different colors within a display period of each image frame, wherein the subpixels having the at least two different colors are within different pixel units of the pixel units.