Multi-band Wake-up (MWU) signal transmission and detection is disclosed. A transmitter determines a MWU signal that indicates availability of one or more sub-bands to a first user in a serving cell, and transmits the MWU signal for the one or more sub-bands in the serving cell. A clear channel assessment (CCA) status on the one or more sub-bands that are available to the first user is carried by the payload of MWU signals. The MWU signal includes a first user identifier that identifies the first user as a targeted receiver of the MWU signal. The first user has priority over a second user to utilize the one or more sub-bands. Correspondingly, a receiver receives a MWU signal for one or more sub-bands in the serving cell, decodes a payload of the MWU signal, and determines availability of the one or more sub-bands to the first user based on the payload of the MWU signal.