A microfluidic system is disclosed, including: a first substrate, a second substrate and a droplet flow channel arranged therebetween; a droplet driving unit configured to drive a droplet to move; a first control circuit electrically connected to the droplet driving unit and configured to input a first driving signal to the droplet driving unit to enable the droplet to move along the predetermined movement trajectory; a droplet detection unit configured to detect the droplet and output a detection signal; a second control circuit electrically connected to the droplet detection unit and configured to receive the detection signal and acquire an actual movement trajectory of the droplet; and a signal adjustment unit configured to compare the actual movement trajectory with the predetermined movement trajectory, and if the actual movement trajectory is different from the predetermined movement trajectory, adjust in real time the first driving signal into a second driving signal.