Provided is a culture medium including fibers aligned in one direction, and a method for producing the same. The culture medium includes: a substrate; a frame body that includes a first surface, a second surface opposite therefrom, and one or more through holes extending from the first surface through the second surface, and that is mounted to the substrate such that the first surface is opposed thereto; a plurality of aligned fibers interposed between the substrate and the first surface; and a bonding portion that bonds the substrate, the plurality of aligned fibers, and the frame body, wherein at least a part of the plurality of aligned fibers is exposed from a first opening that is formed in the first surface by the through hole.