- 专利标题： Compositions and methods for identifying and sorting antigen-specific B cells
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申请号： US16335891申请日： 2017-09-22
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公开(公告)号： US11236153B2公开(公告)日： 2022-02-01
- 发明人: Arash Grakoui , Joseph Marcotrigiano
- 申请人： Emory University , Rutgers, The State University of New Jersey
- 申请人地址： US GA Atlanta; US NJ New Brunswick
- 专利权人： Emory University,Rutgers, The State University of New Jersey
- 当前专利权人： Emory University,Rutgers, The State University of New Jersey
- 当前专利权人地址： US GA Atlanta; US NJ New Brunswick
- 代理机构： Emory Patent Group
- 国际申请： PCT/US2017/052882 WO 20170922
- 国际公布： WO2018/057843 WO 20180329
- 主分类号： A61K39/00
- IPC分类号： A61K39/00 ; A61K39/21 ; C12N7/00 ; A61K39/12 ; C12N15/00 ; C07K16/10 ; C12N15/10 ; C12Q1/686
摘要：
The present disclosure relates to constructs useful in expressing biotinylated monomers and tetramers produced from these monomers. Specifically, the disclosure provides a construct useful in expressing biotinylated antigen monomers, said construct comprising a viral protein of an ectodomain of Hepatitis C Virus (HCV) envelope glycoprotein E2 or Human Immunodeficiency Virus (HIV) gp140. Further disclosed are methods for production and use of these tetramers in identifying and isolating antigen specific B cells and cloning antibodies thereto.
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