- 专利标题： Polypeptides having hydrolytic activity on 1-kestose in the presence of sucrose but lacking sucrase (invertase) activity, polynucleotides encoding same and methods of producting and using same in industrial sucrose production from 1-kestose
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申请号： US15775690申请日： 2016-11-11
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公开(公告)号： US11306337B2公开(公告)日： 2022-04-19
- 发明人: Iuri Estrada Gouvea , Cesar Moises Camilo , Jaime Finguerut , Michael Cook
- 申请人： CTC—Centro de Tecnologia Canavieira S.A.
- 申请人地址： BR Piracicaba
- 专利权人： CTC—Centro de Tecnologia Canavieira S.A.
- 当前专利权人： CTC—Centro de Tecnologia Canavieira S.A.
- 当前专利权人地址： BR Piracicaba
- 代理机构： Smith Tempel Blaha LLC
- 代理商 Laurence P. Colton
- 国际申请： PCT/BR2016/050295 WO 20161111
- 国际公布： WO2017/079818 WO 20170518
- 主分类号： C12P19/12
- IPC分类号： C12P19/12 ; C12P19/14 ; C13B20/00 ; C12N9/24 ; C12P19/02
摘要：
An industrial process using kestose hydrolases to enable the use of sugar mixtures containing 1-kestose in the industrial production of sucrose (as crystallized sugar) by providing a method for the conversion of 1-kestose into sucrose and fructose in a sugar solution, containing kestose and more than 10 mM sucrose (3 g/L), and comprising the enzymatic hydrolysis (preferably using 1-FEH enzymes—EC 3.2.1.153) of 1-kestose. The process further provides a method of producing a polypeptide having 1-kestose hydrolase activity and a composition comprising the polypeptide.
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