- 专利标题: Low oxygen tension enhances endothelial fate of human pluripotent stem cells
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申请号: US15115718申请日: 2014-03-17
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公开(公告)号: US11352606B2公开(公告)日: 2022-06-07
- 发明人: Sharon Gerecht , Sravanti Kusuma
- 申请人: THE JOHNS HOPKINS UNIVERSITY
- 申请人地址: US MD Baltimore
- 专利权人: THE JOHNS HOPKINS UNIVERSITY
- 当前专利权人: THE JOHNS HOPKINS UNIVERSITY
- 当前专利权人地址: US MD Baltimore
- 代理机构: DLA Piper LLP (US)
- 国际申请: PCT/US2014/030616 WO 20140317
- 国际公布: WO2015/119642 WO 20150813
- 主分类号: C12N5/071
- IPC分类号: C12N5/071 ; A61K35/44 ; A61K35/545
摘要:
Low oxygen tension is a critical regulator of the developing or regenerating vasculature. The present invention is based on the determination that low oxygen tension during early stages of early vascular cell (EVC) derivation induces endothelial commitment and maturation of pluripotent stem cells. Inhibition of reactive oxygen species generation during the early stages of differentiation abrogates the endothelial inductive effects of the low oxygen environments. Methods of generating various types of cells from pluripotent stem cells (PSCs) are described, as well as compositions and methods of use thereof. In particular, generation of EVCs, bicellular vascular populations, early endothelial cells (ECs) and pericytes via culture in a low oxygen environment is described.