Invention Grant
- Patent Title: Method of producing three-dimensional tissue having vascular system structure, and three-dimensional tissue including gel having vascular system structure
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Application No.: US15998571Application Date: 2017-01-31
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Publication No.: US11473054B2Publication Date: 2022-10-18
- Inventor: Michiya Matsusaki
- Applicant: OSAKA UNIVERSITY
- Applicant Address: JP Suita
- Assignee: OSAKA UNIVERSITY
- Current Assignee: OSAKA UNIVERSITY
- Current Assignee Address: JP Suita
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P.
- Priority: JPJP2016-027196 20160216
- International Application: PCT/JP2017/003368 WO 20170131
- International Announcement: WO2017/141691 WO 20170824
- Main IPC: C12N5/00
- IPC: C12N5/00 ; A61L27/22 ; A61L27/38 ; A61L27/50 ; C12N5/077 ; C12N5/071
Abstract:
Provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (a) a step for forming a vascular system structure template using a gel; (b) a step for forming a three-dimensional tissue in the vicinity of the template; (c) a step for dissolving the template using a cationic solution; and (d) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells in a void remaining after the dissolution of the template. Also provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (i) a step for forming a vascular system structure template using a gel; (ii) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells on the template; (iii) a step for forming a three-dimensional tissue in the vicinity of the cells seeded above; and (iv) a step for dissolving the template using a cationic solution. Also provided is a three-dimensional tissue comprising a gel which has a vascular system structure.
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