Systems and methods are provided for gathering and segregation of oil extracted from a resource fields with a plurality of disparate sources in relatively close proximity. Instead of assigning a quality and/or destination for crude oil from an extraction source based on the origin of the crude oil, the crude oil can be characterized while still in local storage at or near the wellhead prior to transport to a refinery, a shipping or pipeline terminal, or another type destination for crude oil. Based on the characterization, one or more characteristics of the crude oil can be used to assign the crude oil to one of a plurality of destinations. The crude oil can then be transported to the assigned destination.