A display substrate includes: a base substrate, a drive circuit on the base substrate, an insulating layer on a side of the drive circuit away from the base substrate, and a fingerprint recognition device on a side of the insulating layer away from the base substrate. The fingerprint recognition device includes: a first electrode, a photosensitive layer and a second electrode that are disposed in a stacked manner. The first electrode electrically connects to the drive circuit through a via hole running through the insulating layer; and the first electrode forms a recess in the via hole, and the recess is filled with an insulating material, so that a surface of the first electrode on a side away from the base substrate is a flat surface, and the photosensitive layer is disposed on the flat surface.