The present disclosure relates to a method and a device for generating and transmitting a media file containing three-dimensional (3D) video content. The present disclosure also relates to a method and a device for reconstructing and reproducing three-dimensional video content from a received media file. According to an aspect of an embodiment of the present disclosure, a method of generating a media file containing three-dimensional video content may include inserting, into a media data box in the media file, a plurality of components contained in a two-dimensional video frame in which the three-dimensional video content is compressed; inserting, into a metadata box in the media file, first tracks respectively corresponding to the components and a second track containing metadata; and inserting, into the second track, information about an entry point for each of the plurality of components.