Methods, systems, and media for synchronizing audio and video content on multiple media devices are provided. In some embodiments, the method comprises: receiving, at a media device, an indication of a media content item to be presented using the media device, wherein the media device includes an audio component for presenting audio content associated with the media content item and a video component for presenting video content associated with the media content item; determining that the media device is associated with a group of media devices for presenting the media content item, wherein the group of media devices includes the media device and at least one audio device that presents the audio content associated with the media content item; generating an audio timestamp that controls the presentation of the audio content on the group of media devices; generating a video timestamp that control the presentation of the video content on the group of media devices, wherein the video timestamp is based on the generated audio timestamp; and causing, at the media device, the video content associated with the media content item to be presented using the generated video timestamp and causing the audio content associated with the media content item to be simultaneously presented by the at least one audio device in the group of media devices by transmitting the audio timestamp to the at least one audio device in the group of media devices.