The present invention relates to an electrode for a biosensor for NADH measurement and a manufacturing method therefor. An electrode manufactured by the method according to the present invention enjoys the advantages of stabilizing current flow during an electric polymerization reaction, making the contact angle of the modified material remarkably small to increase the efficiency of surface modification, and being reusable several times. In addition, when applied to a biosensor for NADH measurement, the electrode of the present invention maintains sensitivity and selectivity at a high level without interference and thus easily measures a target of interest even in blood or serum that necessarily requires a pretreatment process due to the existence of a trace amount of a material to be measured. In addition, when applied to a biosensor for NADH measurement, the electrode can measure cell viability in a continuous manner and in real time, which leads to the application thereof to the cell toxicity assay field, and enables the measurement of cell viability in apoptotic cells lacking the mitochondrial function.