An embodiment of the disclosure provides an immunodetection chip, an immunodetection device and a using method. The immunodetection chip includes a substrate and a cover plate. The substrate is disposed opposite to the cover plate to form a detection chamber. One side, facing the cover plate, of the substrate is fixedly provided with substrate antibodies. An inside wall of the detection chamber is provided with a detection member. The detection member is configured to output a corresponding electrical signal while adsorbing biological magnetic beads. The substrate antibodies match with target antigens.