A display substrate including a first display area including a plurality of first sub-pixels, a plurality of second sub-pixels and a plurality of third sub-pixels, wherein the display substrate includes a plurality of first pixel groups each of which includes two third sub-pixels, one first sub-pixel and one second sub-pixel, the two third sub-pixels are arranged adjacent to each other along a first direction, the one first sub-pixel and the one second sub-pixel are adjacent to at least one of the two third sub-pixels, located on both sides of a straight line passing centers of the two third sub-pixels, and arranged along a second direction different from the first direction; a size of the first sub-pixel and the second sub-pixel in the second direction is smaller than a size of the first sub-pixel and the second sub-pixel in the first direction, respectively.