The disclosure provides a rock stratum grouting water blocking test device and method under a mining coupling condition, and relates to the technical field of permeability testing. The device includes a solution box, a test box, a pressing plate replacement box, a grouting pump, a recovery pool and a water pump, the solution box is connected with the test box through a liquid injection pipe, the bottom of the test box is connected with the pressing plate replacement box, the pressing plate replacement box is connected with a bottom plate, and the bottom plate is connected with a control panel. The device can be used for testing the influence of the single-factor or multi-factor coupling action of temperature, water quality, water pressure, water flow and grouting pressure on the grouting effect.