A method for producing an anisotropic conductive film, which includes mixing a base polymer with an initiator and a first solvent to obtain a first mixture, dispersing conductive microspheres in an alcohol or a second solvent containing a surfactant to obtain a second mixture, mixing the first mixture with a monomer, a binder, and the second mixture to obtain a third mixture, and laminating the third mixture on a carrying film, followed by drying, so as to obtain the anisotropic conductive film. An anisotropic conductive film produced by the method and a composition for forming the anisotropic conductive film are also disclosed.